A functional single-molecule binding assay via force spectroscopy
نویسندگان
چکیده
منابع مشابه
A force-spectroscopy-based single-molecule metal-binding assay.
Quantifying metal-binding by force: A quantitative single-molecule force-spectroscopy-based assay is developed to measure the binding affinity of metal ions to proteins. The method uses the unfolding force of a protein as a direct probe to distinguish the apo and metal-ion-bound forms of that protein and quantify the partitioning between the two forms (see figure).
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The eukaryotic signaling protein calmodulin (CaM) can bind to more than 300 known target proteins to regulate numerous functions in our body in a calcium-dependent manner. How CaM distinguishes between these various targets is still largely unknown. Here, we investigate fluctuations of the complex formation of CaM and its target peptide sequences using single-molecule force spectroscopy by AFM....
متن کاملOn artifacts in single-molecule force spectroscopy.
In typical force spectroscopy experiments, a small biomolecule is attached to a soft polymer linker that is pulled with a relatively large bead or cantilever. At constant force, the total extension stochastically changes between two (or more) values, indicating that the biomolecule undergoes transitions between two (or several) conformational states. In this paper, we consider the influence of ...
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The deflection of a cantilever is measured using a laser beam and position-sensing detector to obtain force and displacement for both imaging and force spectroscopy. In force spectroscopy, the cantilever is attached to one end of the protein or DNA sample while the other end is immobilized on the surface. The force and position of the tip are measured as the surface is moved away from the tip, ...
متن کاملDynamic Force Spectroscopy of Single Molecule
The direct measurement of forces between individual receptor-ligand complexes is an emerging new field. With the atomic force microscope (AFM) it has been possible to probe the forces between a range of biological molecules, including complementary oligo-nucleotides, proteins and carbohydrate interactions. To date, the force required to rupture these single ligandreceptor interactions is common...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2007
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0705367104